Identification of key residues for carbonic anhydrase isoenzymes: use of sequence and crystallographic data
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چکیده
منابع مشابه
Gene expression for carbonic anhydrase isoenzymes in human nasal mucosa.
Carbonic anhydrase (CA) is physiologically important in the reversible hydration reaction of CO(2); it is expressed in a number of isoforms (CA I-XIV) with varying degrees of enzymatic activity. In nasal chemesthesis, CA inhibition decreases the electrophysiologic response to CO(2), a common irritant test compound. CA enzymatic activity has been demonstrated in the human nasal mucosa using enzy...
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We compare the effect of buffers on the catalysis by bovine carbonic anhydrase, human carbonic anhydrase C (HCA 0, and human carbonic anhydrase B (HCA B) of two types of ‘“0 exchange. Type I, resulting from the hydrationdehydration reaction, is the exchange of IsO between CO, and water. Type II is the exchange of IsO between ‘*Ccontaining and ‘“C-containing species of COZ. Imidazole, 2,4-lutidi...
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S. ALIAKBAR,*t P. R. BROWN,* E. JAUNIAUX,t D. E. BIDWELLS and K. H. NlCOLAIDESt *Department of Biochemistry, King’s College London, Strand, London WC2R 2L.S. tllarris Birthright Research Centre for Fetal Medicine, Department of Obstetrics and Gynaecology, King’s College Hospital, Denmark Hill, London SE5 8RX, and $ Nufield Laboratories of Comparative Medicine, The Zoological Society of London, ...
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The nucleotide sequence of a clone containing mouse carbonic anhydrase (CA) eDNA in pBR322 has been determined. The cloned eDNA contains all of the coding region except for nueleotides specifying the first eight amino acids, and all of the 3' noncoding region, which consists of 700 nucleotides. A eDNA clone was identified which contains an additional 54 bp at the 5' end, so that the complete am...
متن کاملCapsaicin: a potent inhibitor of carbonic anhydrase isoenzymes.
Carbonic anhydrase (CA, EC 4.2.1.1) is a zinc containing metalloenzyme that catalyzes the rapid and reversible conversion of carbon dioxide (CO2) and water (H2O) into a proton (H+) and bicarbonate (HCO3-) ion. On the other hand, capsaicin is the main component in hot chili peppers and is used extensively used in spices, food additives and drugs; it is responsible for their spicy flavor and pung...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 1993
ISSN: 0108-7673
DOI: 10.1107/s0108767378095616